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Induction of various bloodbrain barrier properties in non-neuronal endothelial cells by close apposition to co-cultured astrocytes

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Cell transfection When HUVECs confluency met approximately 6070%, cells in 6-well plates were transfected with plasmid DNA or siRNA for overexpression or knockdown of GSDMD using Lipofectamine TM 3000 (Invitrogen)